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NLD410/413/423Culture Plate

The difference and choice of flat bottom and round bottom (U-type and V-type) culture plates
Different shapes of culture plates have different uses. For culturing cells, a flat bottom is usually used, which is convenient for observation under the microscope, has a clear bottom area, and the height of the cell culture liquid is relatively consistent. Therefore, when doing experiments such as MTT, whether it is adherent or suspended cells, a flat bottom plate is generally used. A flat-bottomed culture plate must be used to measure the absorbance. Pay special attention to the material. The label "Tissue Culture (TC) Treated" is for cell culture.U-shaped or V-shaped plates are generally used when some special requirements are required. For example, in immunology, when two different lymphocytes are mixed culture, the two need to contact each other to stimulate each other. At this time, U-shaped plates are generally used because the cells will gather in a small area due to gravity. The round bottom culture plate will also be used for the experiment of isotope incorporation. It is necessary to use a cell harvester to collect the cell culture, such as "mixed lymphocyte culture". The V-shaped plate is commonly used for cell killing and immunological hemagglutination experiments. The cell killing experiment can also be replaced by a U-shaped plate (after adding cells, centrifugation at low speed).
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